7RCA
Crystal structure of Aro2p chorismate synthase from Candida lusitaniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-10-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 94.086, 94.086, 84.618 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.830 - 2.260 |
R-factor | 0.205 |
Rwork | 0.200 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r53 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.600 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.310 |
High resolution limit [Å] | 2.260 | 2.270 |
Rmerge | 0.082 | 1.147 |
Rpim | 0.023 | 0.357 |
Number of reflections | 17965 | 726 |
<I/σ(I)> | 29.8 | 1.1 |
Completeness [%] | 98.2 | 80.2 |
Redundancy | 13.7 | 9.9 |
CC(1/2) | 1.000 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.1 M NaCl, 0.1 M HEPES pH 7.2, 1.6 M ammonium sulfate |