7RBP

Medicago truncatula D-1-piperideine-2-carboxylic acid reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	80.140, 83.665, 113.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.410 - 2.100
R-factor	0.2529
Rwork	0.252
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bv9
RMSD bond length	0.006
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.410	2.179
High resolution limit [Å]	2.100	2.104
Rmerge	0.014	0.658
Rmeas	0.014	0.658
Rpim	0.014	0.685
Number of reflections	45056	4425
<I/σ(I)>	16.22	1.22
Completeness [%]	98.8	99.68
Redundancy	2	2
CC(1/2)	1.000	0.291

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.25 M Ammonium Tartrate Dibasic, 24% PEG 3350