7RBM
Human DNA polymerase beta crosslinked complex, 60 s Ca to Mn exchange
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.726, 82.115, 54.203 |
Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
Resolution | 31.970 - 2.210 |
R-factor | 0.2088 |
Rwork | 0.204 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rpy |
RMSD bond length | 0.003 |
RMSD bond angle | 1.118 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.670 | 46.670 | 2.270 |
High resolution limit [Å] | 2.210 | 9.090 | 2.210 |
Rmerge | 0.050 | 0.022 | 0.528 |
Rmeas | 0.059 | 0.026 | 0.638 |
Rpim | 0.032 | 0.014 | 0.353 |
Total number of observations | 68041 | 928 | 5265 |
Number of reflections | 20253 | 295 | 1694 |
<I/σ(I)> | 12.8 | 40.1 | 2.2 |
Completeness [%] | 98.0 | 93.9 | 93.9 |
Redundancy | 3.4 | 3.1 | 3.1 |
CC(1/2) | 0.999 | 0.999 | 0.805 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |