7RBL
Human DNA polymerase beta crosslinked complex, 60 s Ca to Mg exchange
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.306, 81.394, 55.534 |
Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
Resolution | 40.730 - 1.980 |
R-factor | 0.1902 |
Rwork | 0.188 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rpy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.512 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.840 | 47.840 | 2.030 |
High resolution limit [Å] | 1.980 | 9.070 | 1.980 |
Rmerge | 0.066 | 0.043 | 0.524 |
Rmeas | 0.072 | 0.046 | 0.574 |
Rpim | 0.027 | 0.017 | 0.230 |
Total number of observations | 198825 | 2165 | 11756 |
Number of reflections | 29288 | 322 | 1985 |
<I/σ(I)> | 15.1 | 35.8 | 3.2 |
Completeness [%] | 98.6 | 97.7 | 95.7 |
Redundancy | 6.8 | 6.7 | 5.9 |
CC(1/2) | 0.999 | 0.998 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |