7RBH
Human DNA polymerase beta crosslinked ternary complex 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.670, 80.732, 55.705 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 48.270 - 1.750 |
| R-factor | 0.1816 |
| Rwork | 0.179 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.786 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.270 | 48.270 | 1.780 |
| High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
| Rmerge | 0.037 | 0.022 | 0.469 |
| Rmeas | 0.044 | 0.026 | 0.557 |
| Rpim | 0.023 | 0.014 | 0.297 |
| Total number of observations | 144039 | 1096 | 7641 |
| Number of reflections | 41838 | 319 | 2267 |
| <I/σ(I)> | 17.1 | 46.3 | 2.5 |
| Completeness [%] | 97.0 | 97.2 | 97.4 |
| Redundancy | 3.4 | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.998 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
