7RBE
Human DNA polymerase beta crosslinked binary complex - A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.585, 79.480, 54.906 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 44.180 - 1.890 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.602 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.180 | 44.180 | 1.940 |
| High resolution limit [Å] | 1.890 | 9.080 | 1.890 |
| Rmerge | 0.058 | 0.027 | 0.517 |
| Rmeas | 0.068 | 0.033 | 0.622 |
| Rpim | 0.035 | 0.018 | 0.339 |
| Total number of observations | 128492 | 828 | 5230 |
| Number of reflections | 34956 | 271 | 1778 |
| <I/σ(I)> | 10.4 | 22.3 | 1.4 |
| Completeness [%] | 96.8 | 79.2 | 78.5 |
| Redundancy | 3.7 | 3.1 | 2.9 |
| CC(1/2) | 0.998 | 0.997 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
