7RAZ
Small conductance mechanosensitive channel MscS
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Staining material | 2% uranyl acetate |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 500000 |
| Software | RELION,PHENIX,Leginon,CTFFIND,UCSF Chimera,Coot |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
