7RAW

Domain 1 of Starch adherence system protein 20 (Sas20) from Ruminococcus bromii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-10
Detector	RAYONIX MX300-HS
Wavelength(s)	0.979
Spacegroup name	I 21 3
Unit cell lengths	130.032, 130.032, 130.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.1802
Rwork	0.177
R-free	0.21420
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.120	30.000	2.140
High resolution limit [Å]	2.099	5.690	2.100
Rmerge	0.071	0.032	2.311
Rmeas	0.074	0.033	2.406
Rpim	0.019	0.009	0.665
Number of reflections	21541	1140	1051
<I/σ(I)>	8.7
Completeness [%]	100.0	100	100
Redundancy	14.8	14.1	13
CC(1/2)		0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	0.024M 1,6-Hexanediol; 0.024M 1-Butanol 0.024M 1,2-Propanediol; 0.024M 2-Propanol; 0.024M 1,4-Butanediol; 0.024M 1,3-Propanediol; 0.1M Imidazole; 0.1M MES monohydrate; 20% PEG 500 MME; 10% PEG 20000