7RAO

Structure of M66I mutant of disulfide stabilized HIV-1 CA hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-07-01
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 6
Unit cell lengths	158.962, 158.962, 57.711
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.220 - 2.290
R-factor	0.2169
Rwork	0.215
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h47
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.230	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.039	0.229
Number of reflections	37715	3732
<I/σ(I)>	20.2	2
Completeness [%]	99.7	99.3
Redundancy	2	2
CC(1/2)	0.998	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.125-0.35M Sodium Iodide, 2-8% Peg 3350, 6% glycerol, 0.1M sodium cacodylate pH 6.5