7RA7

Crystal structure of rabbit anti-HIV Fab 11A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03315
Spacegroup name	P 1 21 1
Unit cell lengths	71.359, 73.877, 86.446
Unit cell angles	90.00, 97.82, 90.00

Refinement procedure

Resolution	45.900 - 2.200
R-factor	0.2168
Rwork	0.215
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model
RMSD bond length	0.002
RMSD bond angle	0.562
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.185	1.200
Rmeas	0.201	1.300
Rpim	0.078	0.550
Number of reflections	44732	2195
<I/σ(I)>	9	1
Completeness [%]	98.7	96.5
Redundancy	6.4	5.2
CC(1/2)	0.860	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	0.1M sodium acetate, 0.2M lithium sulfate, 30% PEG8000