7R9W
LC3A in complex with Fragment 1-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 |
| Unit cell lengths | 94.761, 94.761, 32.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.690 - 1.750 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wal |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.690 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.038 | 0.528 |
| Number of reflections | 14949 | 797 |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Sodium formate, 20% PEG 3350 |
