7R9D
Crystal structure of Nb_0 in complex with Fab_8D3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.510, 60.287, 77.244 |
Unit cell angles | 90.00, 109.34, 90.00 |
Refinement procedure
Resolution | 28.450 - 1.830 |
R-factor | 0.1808 |
Rwork | 0.180 |
R-free | 0.21060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Fab with similar sequence |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (v.1.12) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.068 | 0.550 |
Number of reflections | 49251 | 3189 |
<I/σ(I)> | 16.46 | |
Completeness [%] | 99.3 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Ammonium formate, 20-22 % w/v PEG 3350 |