7R8H

Crystal structure of Pseudooceanicola lipolyticus Argonaute bound to 5' p guide DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 2 3
Unit cell lengths	199.823, 199.823, 199.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.680 - 2.540
R-factor	0.202
Rwork	0.200
R-free	0.23860
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.540	2.550
Rpim	0.027
Number of reflections	43544	2179
<I/σ(I)>	28.8	1
Completeness [%]	100.0	100
Redundancy	19.9
CC(1/2)	1.000	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	100 mM Tris-HCl, pH 8.0, 8% PEG 8,000