7R84

Structure of mouse BAI1 (ADGRB1) TSR3 domain in P21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	40.572, 60.784, 44.420
Unit cell angles	90.00, 109.67, 90.00

Refinement procedure

Resolution	41.827 - 1.336
R-factor	0.1789
Rwork	0.177
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Complex structure of BAI1-RTN4R
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.830	41.830	1.360
High resolution limit [Å]	1.336	7.320	1.340
Rmerge	0.065	0.047	1.457
Rmeas	0.071	0.051	1.621
Rpim	0.027	0.019	0.695
Total number of observations		1968	8079
Number of reflections	44014	305	1557
<I/σ(I)>	11.9	33	0.9
Completeness [%]	95.5	99.8	68.4
Redundancy	6.5	6.5	5.2
CC(1/2)	0.999	0.998	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M acetate, pH 4.6, 2M sodium formate