7R4W
Single stranded DNA binding protein SSB M5 from Fervidobacterium gondwanense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-25 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 62.390, 62.390, 172.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.130 - 2.300 |
| R-factor | 0.1902 |
| Rwork | 0.187 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vw9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.091 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.130 | 43.100 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.131 | 0.035 | 5.500 |
| Rmeas | 0.134 | 0.036 | 5.603 |
| Rpim | 0.027 | 0.008 | 1.061 |
| Total number of observations | 3616 | 20669 | |
| Number of reflections | 7947 | 171 | 762 |
| <I/σ(I)> | 17.6 | 61.8 | 1 |
| Completeness [%] | 99.4 | 98.1 | 99.5 |
| Redundancy | 25.8 | 21.1 | 27.1 |
| CC(1/2) | 0.999 | 1.000 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 1 M Diammonium phosphate, 0.1 M sodium acetate pH 4.5 |
