7R4W
Single stranded DNA binding protein SSB M5 from Fervidobacterium gondwanense
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-25 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 62.390, 62.390, 172.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.130 - 2.300 |
R-factor | 0.1902 |
Rwork | 0.187 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vw9 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.091 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MrBUMP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.130 | 43.100 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.131 | 0.035 | 5.500 |
Rmeas | 0.134 | 0.036 | 5.603 |
Rpim | 0.027 | 0.008 | 1.061 |
Total number of observations | 3616 | 20669 | |
Number of reflections | 7947 | 171 | 762 |
<I/σ(I)> | 17.6 | 61.8 | 1 |
Completeness [%] | 99.4 | 98.1 | 99.5 |
Redundancy | 25.8 | 21.1 | 27.1 |
CC(1/2) | 0.999 | 1.000 | 0.521 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 1 M Diammonium phosphate, 0.1 M sodium acetate pH 4.5 |