7R4U

Apoform of FtrA/P19 from Rubrivivax gelatinosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979336
Spacegroup name	P 21 21 21
Unit cell lengths	59.340, 79.970, 82.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.250 - 1.230
R-factor	0.171
Rwork	0.170
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7r3p
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.250	1.300
High resolution limit [Å]	1.230	1.230
Rmerge	0.067	2.000
Number of reflections	114401	17537
<I/σ(I)>	18.9
Completeness [%]	98.9
Redundancy	12.77
CC(1/2)	1.000	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	292	2M AS, 0.1 M NaCitrate