7R49
Crystal structure of the L. plantarum acyl carrier protein synthase (AcpS)in complex with D-alanyl carrier protein (DltC1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-07 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.537, 94.888, 98.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.790 - 1.880 |
R-factor | 0.2176 |
Rwork | 0.216 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f80 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.790 | 1.920 |
High resolution limit [Å] | 1.880 | 1.880 |
Rpim | 0.063 | 0.569 |
Number of reflections | 52945 | 3096 |
<I/σ(I)> | 9.1 | 1.4 |
Completeness [%] | 99.3 | |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, Sodium Malonate |