7R3P

Apoform of the periplasmic FtrA/P19 protein from Rubrivivax gelatinosus (His-tag)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	59.160, 80.300, 82.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.120 - 1.400
R-factor	0.18
Rwork	0.179
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzl
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.120	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.086
Number of reflections	78363	12299
<I/σ(I)>	15.9
Completeness [%]	99.7
Redundancy	13
CC(1/2)	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	292	2 M AS, 0.1 M citrate sodium