7R2Z
Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with ATP and ADP resulting from long ATP soak
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 60.802, 60.802, 78.435 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.450 - 2.400 |
| R-factor | 0.18756 |
| Rwork | 0.186 |
| R-free | 0.21331 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5o3p |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.084 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.450 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.058 | 0.784 |
| Number of reflections | 12684 | 2032 |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 7.23 | |
| CC(1/2) | 0.999 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M SODIUM ACETATE, 0.1 M HEPES PH 7.5, 20 % (W/V) PEG3000 |
