7R2X
Paradendryphiella salina PL8 mannuronate-specific alginate lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 31 |
Unit cell lengths | 108.890, 108.890, 150.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.150 - 3.350 |
R-factor | 0.2092 |
Rwork | 0.207 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alpha Fold model |
RMSD bond length | 0.002 |
RMSD bond angle | 0.431 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | ISOLDE |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.150 | 3.470 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 28559 | 2833 |
<I/σ(I)> | 0.99 | |
Completeness [%] | 99.4 | |
Redundancy | 3.5 | |
CC(1/2) | 0.990 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350 and 0.1 M Bis-Tris pH 5.5 |