7R2T

SYNJ2BP complex with a phosphorylated Vangl2 peptide at the P-1 position.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	60.100, 61.380, 145.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.888 - 2.500
R-factor	0.2178
Rwork	0.217
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jik
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.888	46.888	2.570
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.083	0.027	1.322
Rmeas	0.086	0.029	1.380
Total number of observations	247294
Number of reflections	19104	261	1334
<I/σ(I)>	20.45	55.78	1.76
Completeness [%]	98.8	95.3	96.5
Redundancy	12.945	9.977	12.142
CC(1/2)	1.000	1.000	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Ammonium sulfate, 0.1 M Sodium formate, 0.1 M HEPES 7.0, 25 % v/v PEG Smear Broad