7R0K

Crystal structure of Polymerase I from phage G20c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	309.770, 98.011, 77.614
Unit cell angles	90.00, 98.90, 90.00

Refinement procedure

Resolution	153.020 - 2.972
R-factor	0.2179
Rwork	0.216
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bwm
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.020	3.254
High resolution limit [Å]	2.972	2.972
Rmerge	0.095	1.379
Rmeas	0.104	1.485
Rpim	0.039	0.548
Number of reflections	32681	1634
<I/σ(I)>	12.3
Completeness [%]	68.9	14.6
Redundancy	7.1
CC(1/2)	0.999	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1.3 M AmSO4 0.1 M Tris PH 8 0.05 M Li2SO4 0.02 M MgCl2