7R0D
Structure of NUDT15 in complex with Geranyl monophosphate
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97623 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.642, 48.269, 135.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.500 - 1.700 |
Rwork | 0.184 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lpg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.715 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.640 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 34417 | 1759 |
<I/σ(I)> | 17.8 | |
Completeness [%] | 99.7 | |
Redundancy | 11.3 | |
CC(1/2) | 0.998 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris pH 8.5, 0.2 M sodium acetate, 30 % PEG4000 |