7R07

Abortive infection DNA polymerase AbiK from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0064
Spacegroup name	P 62 2 2
Unit cell lengths	200.990, 200.990, 570.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.627 - 3.100
R-factor	0.19
Rwork	0.189
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292120632
RMSD bond length	0.002
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.627	3.290
High resolution limit [Å]	3.100	3.100
Number of reflections	123156	19473
<I/σ(I)>	19.7
Completeness [%]	99.4
Redundancy	40.82
CC(1/2)	1.000	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% Jeffamine M-600, 0.1 M MES (pH 6.5), and 0.05 M cesium chloride