7QZS
Crystal structure of mouse CNPase catalytic domain, G324D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-20 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.968 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 68.630, 45.190, 61.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.740 - 1.800 |
R-factor | 0.1997 |
Rwork | 0.195 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmi |
RMSD bond length | 0.011 |
RMSD bond angle | 1.149 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 0.150 | 2.927 |
Number of reflections | 17959 | 1300 |
<I/σ(I)> | 8 | 0.7 |
Completeness [%] | 98.1 | 98.8 |
Redundancy | 7.8 | 7.7 |
CC(1/2) | 0.997 | 0.319 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 30% (v/v) Jeffamine ED-2003 and 0.1M HEPES (pH 7.0) |