7QZP
Identification and characterization of an RRM-containing, ELAV-like, RNA binding protein in Acinetobacter Baumannii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-02 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 |
Unit cell lengths | 69.560, 69.560, 32.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.870 - 1.650 |
R-factor | 0.2131 |
Rwork | 0.212 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fxl |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20rc1_4392) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 8220 | 857 |
<I/σ(I)> | 18.76 | 1.7 |
Completeness [%] | 87.1 | |
Redundancy | 11.7 | |
CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate trihydrate, 3 M sodium chloride |