7QZD
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikF with an engineered HMA domain of Pikp-1 (Pikp-SNK-EKE) from rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.381, 80.782, 103.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.830 - 2.200 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a6w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 103.800 | 103.800 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.097 | 0.035 | 0.751 |
| Rmeas | 0.105 | 0.039 | 0.814 |
| Rpim | 0.038 | 0.015 | 0.302 |
| Total number of observations | 195704 | 2745 | 16870 |
| Number of reflections | 28553 | 457 | 2512 |
| <I/σ(I)> | 11.5 | 32.5 | 2.2 |
| Completeness [%] | 97.4 | 93.7 | 99 |
| Redundancy | 6.9 | 6 | 6.7 |
| CC(1/2) | 0.998 | 0.999 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | NULL |
