7QZ3
The structure of T. forsythia NanH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.740, 79.740, 349.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.863 - 1.970 |
| Rwork | 0.230 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bbw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.350 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.863 | 2.010 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 80616 | 4386 |
| <I/σ(I)> | 21.8 | 1.3 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.81 | |
| CC(1/2) | 1.000 | 0.472 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 7% PEG6000 0.1M HEPES |
