7QYX
Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-24 (R207A, D210S, S211T)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.77490 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.258, 74.141, 147.466 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.335 - 1.850 |
Rwork | 0.198 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zal |
RMSD bond length | 0.011 |
RMSD bond angle | 1.568 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.335 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.119 | 2.141 |
Rmeas | 0.118 | 1.852 |
Number of reflections | 48066 | 7553 |
<I/σ(I)> | 15.16 | 1.75 |
Completeness [%] | 98.4 | 97.7 |
Redundancy | 13.1 | 12.4 |
CC(1/2) | 0.999 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 6K; 15% PEG 400; 0.2M MgCl2 in 0.1 Tris-HCl pH 8.5 |