7QYX

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-24 (R207A, D210S, S211T)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.77490
Spacegroup name	P 21 21 21
Unit cell lengths	50.258, 74.141, 147.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.335 - 1.850
Rwork	0.198
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.011
RMSD bond angle	1.568
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.335	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.119	2.141
Rmeas	0.118	1.852
Number of reflections	48066	7553
<I/σ(I)>	15.16	1.75
Completeness [%]	98.4	97.7
Redundancy	13.1	12.4
CC(1/2)	0.999	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 6K; 15% PEG 400; 0.2M MgCl2 in 0.1 Tris-HCl pH 8.5