7QYR
Crystal structure of RimK from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.898, 153.586, 138.539 |
| Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
| Resolution | 44.450 - 2.400 |
| R-factor | 0.2126 |
| Rwork | 0.212 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iwx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.327 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.100 | 45.100 | 2.440 |
| High resolution limit [Å] | 2.400 | 13.150 | 2.400 |
| Rmerge | 0.108 | 0.036 | 2.049 |
| Rmeas | 0.116 | 0.040 | 2.219 |
| Rpim | 0.044 | 0.016 | 0.845 |
| Total number of observations | 3857 | 35144 | |
| Number of reflections | 105678 | 653 | 5171 |
| <I/σ(I)> | 10.9 | 40.7 | 0.9 |
| Completeness [%] | 99.3 | 96.2 | 98.8 |
| Redundancy | 6.9 | 5.9 | 6.8 |
| CC(1/2) | 0.999 | 0.998 | 0.438 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | NULL |
