7QYQ
Crystal structure of a DyP-type peroxidase from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 141.951, 141.951, 177.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.051 - 2.599 |
| R-factor | 0.2198 |
| Rwork | 0.219 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iiz |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.051 | 2.760 |
| High resolution limit [Å] | 2.599 | 2.600 |
| Rpim | 0.054 | 0.381 |
| Number of reflections | 64014 | 12914 |
| <I/σ(I)> | 9.49 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.3 | |
| CC(1/2) | 0.996 | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M magnesium formate dihydrate and 20 % w/v PEG 3350 |
