7QYM
Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-18 (R207V, D210P, S211W)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.77490 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.552, 75.006, 146.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.896 - 1.200 |
Rwork | 0.127 |
R-free | 0.15340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zal |
RMSD bond length | 0.013 |
RMSD bond angle | 1.727 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.896 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.085 | 1.786 |
Rmeas | 0.088 | 1.857 |
Number of reflections | 167749 | 8018 |
<I/σ(I)> | 14.2 | 1.7 |
Completeness [%] | 98.1 | 95.7 |
Redundancy | 13.7 | 13.2 |
CC(1/2) | 0.999 | 0.556 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 6K; 15% PEG 400; 0.2M MgCl2 in 0.1 Tris-HCl pH 8.5 |