7QYL

ATAD2 in complex with FragLite23

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	79.521, 79.521, 137.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.870 - 1.440
Rwork	0.220
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dai
RMSD bond length	0.015
RMSD bond angle	2.121
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.870	68.870	1.470
High resolution limit [Å]	1.440	7.750	1.440
Rmerge	0.058	0.037	3.990
Rmeas	0.060	0.039	4.413
Rpim	0.018	0.012	1.868
Number of reflections	47120	355	2331
<I/σ(I)>	20.5
Completeness [%]	99.9
Redundancy	19.6	15.3	10.5
CC(1/2)	1.000	0.999	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M BisTris pH 6-7, 1.7-2.1M Ammonium sulphate