7QY8
The structure of T. forsythia NanH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.059, 80.059, 349.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.901 - 2.060 |
Rwork | 0.245 |
R-free | 0.27860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bbw |
RMSD bond length | 0.004 |
RMSD bond angle | 1.306 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.901 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.158 | 2.964 |
Number of reflections | 71921 | 3491 |
<I/σ(I)> | 14.8 | 1.4 |
Completeness [%] | 100.0 | |
Redundancy | 25.6 | |
CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 7% PEG6000 0.1M HEPES |