7QY6

Structure of E.coli Class 2 L-asparaginase EcAIII, wild type (WT EcAIII)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97000
Spacegroup name	P 21 21 21
Unit cell lengths	50.478, 75.347, 147.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.236 - 1.650
Rwork	0.193
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.012
RMSD bond angle	1.694
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.350	1.640
High resolution limit [Å]	1.550	1.550
Rmerge	0.167	1.390
Rmeas	0.158	1.237
Number of reflections	83105	13084
<I/σ(I)>	9.36	1.62
Completeness [%]	99.5	98
Redundancy	12.2
CC(1/2)	0.997	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 6000, 0.2 M MgCl2 in 0.1M Tris-HCl pH 8.5