7QXQ
Coelenteramide-bound Renilla-type luciferase (AncFT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.224, 83.332, 102.017 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 44.891 - 2.251 |
| R-factor | 0.1749 |
| Rwork | 0.172 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s97 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 75744 | 4404 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.997 | 0.594 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | magnesium acetate, PEG 3350 |
