7QX8

Crystal structure of serine hydroxymethyltransferase, isoform 7 from Arabidopsis thaliana (SHM7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	199.832, 123.955, 290.536
Unit cell angles	90.00, 93.17, 90.00

Refinement procedure

Resolution	58.000 - 2.740
R-factor	0.1875
Rwork	0.187
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pzz
RMSD bond length	0.010
RMSD bond angle	1.587
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.000	3.070
High resolution limit [Å]	2.740	2.740
Rmerge	0.092	0.550
Rmeas	0.106	0.627
Number of reflections	121503	6069
<I/σ(I)>	10.9	2.9
Completeness [%]	65.8	65.3
Redundancy	4	4.36
CC(1/2)	0.997	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	75 mM magnesium chloride, 75 mM sodium citrate tribasic, 0.1 M Bis-Tris pH 6.0, 18 % v/v PEG Smear Broad (based on G8 condition of the BCS screen, Molecular Dimensions). Cryoprotection was obtained by supplementation of the original condition with 25% ethylene glycol.