7QX7
mosquitocidal Cry11Aa-F17Y determined at pH 7 from naturally-occurring nanocrystals by Serial femtosecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | EUROPEAN XFEL BEAMLINE SPB/SFX |
Synchrotron site | European XFEL |
Beamline | SPB/SFX |
Temperature [K] | 300 |
Detector technology | PIXEL |
Collection date | 2019-09-26 |
Detector | AGIPD |
Wavelength(s) | 1.33 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 57.720, 155.390, 171.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.170 - 3.400 |
R-factor | 0.2144 |
Rwork | 0.212 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292119069 |
Data reduction software | CrystFEL (0.8.0) |
Data scaling software | CrystFEL (0.8.0) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.170 | 3.480 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 10990 | 707 |
<I/σ(I)> | 6.31 | |
Completeness [%] | 99.6 | |
Redundancy | 265.7 | |
CC(1/2) | 0.960 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | IN CELL | 7 | 300 | In cell crystallization by recombinant expression in a Bacillus thuringiensis serovar israelensis strain devoid of its pBt plasmid. |