7QWV

Crystal structure of the REC114-TOPOVIBL complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 61 2 2
Unit cell lengths	108.729, 108.729, 82.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	94.160 - 2.260
R-factor	0.2274
Rwork	0.226
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hfg
RMSD bond length	0.005
RMSD bond angle	1.365
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.160	2.348
High resolution limit [Å]	2.260	2.260
Number of reflections	11909	596
<I/σ(I)>	23.9	1.37
Completeness [%]	84.4	38.9
Redundancy	18
CC(1/2)	0.999	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	1.6M MgSO4, 0.1 M MES (pH 6.5), 10% dioxane