7QWJ

Crystal structure of Glc7 phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	38.284, 83.294, 96.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 1.650
R-factor	0.2216
Rwork	0.219
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.063	1.879
High resolution limit [Å]	1.650	1.650
Rmerge	0.071	1.034
Rmeas	0.074	0.939
Rpim	0.020	0.357
Number of reflections	22215	1112
<I/σ(I)>	19.7	1.8
Completeness [%]	94.2
Redundancy	12.9
CC(1/2)	1.000	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	20% PEG 4,000, 200 mM MgCl2 and 100 mM TrisHCl pH 8.5