7QWC
CC-Type1-(UbUc)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 41 |
| Unit cell lengths | 39.833, 39.833, 73.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.860 - 1.690 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.333 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.860 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.009 | 1.694 |
| Rmeas | 0.092 | 1.791 |
| Rpim | 0.025 | 0.566 |
| Number of reflections | 12804 | 1265 |
| <I/σ(I)> | 13.32 | 0.46 |
| Completeness [%] | 99.8 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.999 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5 mM peptide, 0.5 M Sodium acetate trihydrate, 50 mM Sodium HEPES, at pH 7.5 |
