7QW5
Adenine-specific DNA methyltransferase M.BseCI complexed with AdoHcy and cognate unmethylated DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 87.040, 87.040, 156.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.490 - 2.300 |
| R-factor | 0.1986 |
| Rwork | 0.197 |
| R-free | 0.22380 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.731 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.910 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 38301 | 3358 |
| <I/σ(I)> | 22.8 | 2 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | PEG 400, Tris-HCl, KCl, magnesium chloride |
