7QW4
Pden_5119 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 134.510, 136.376, 212.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.750 - 3.100 |
| R-factor | 0.3202 |
| Rwork | 0.320 |
| R-free | 0.32120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.094 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 3.140 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.310 | |
| Rmeas | 0.438 | |
| Rpim | 0.060 | |
| Number of reflections | 65429 | 3022 |
| <I/σ(I)> | 9.7 | 2.1 |
| Completeness [%] | 90.2 | 57.6 |
| Redundancy | 2 | |
| CC(1/2) | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 303.15 | 50 mM trisodium phosphate, 300 mM sodium chloride, 300 mM imidazole, pH 8.0 |
