7QVD
X-ray structure of the lytic transglycosylase SltB2 from Pseudomonas aeruginosa
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.966, 42.395, 93.579 |
Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
Resolution | 46.560 - 1.700 |
Rwork | 0.164 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5anz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.560 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.086 | 1.072 |
Rpim | 0.036 | 0.435 |
Number of reflections | 36814 | 1926 |
<I/σ(I)> | 13 | 2.3 |
Completeness [%] | 99.0 | 99.3 |
Redundancy | 6.6 | 6.9 |
CC(1/2) | 0.998 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% Polyethylene Glycol 4000, 0.1 M sodium acetate and 8% 2-propanol |