7QVB

Crystal structure of the DNA-binding protein DdrC from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-14
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9660
Spacegroup name	P 32 2 1
Unit cell lengths	111.721, 111.721, 105.187
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.251 - 2.500
Rwork	0.183
R-free	0.24650
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.233
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.084	2.012
Rmeas	0.099	2.368
Rpim	0.051	1.219
Number of reflections	26653	2978
<I/σ(I)>	12.6	1.1
Completeness [%]	99.9	99.8
Redundancy	6.4	6.4
CC(1/2)	0.999	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris pH8.0, 1.2 M ammonium sulfate