7QVB
Crystal structure of the DNA-binding protein DdrC from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-14 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9660 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.721, 111.721, 105.187 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.251 - 2.500 |
Rwork | 0.183 |
R-free | 0.24650 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 1.233 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.340 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.084 | 2.012 |
Rmeas | 0.099 | 2.368 |
Rpim | 0.051 | 1.219 |
Number of reflections | 26653 | 2978 |
<I/σ(I)> | 12.6 | 1.1 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.4 | 6.4 |
CC(1/2) | 0.999 | 0.479 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris pH8.0, 1.2 M ammonium sulfate |