7QV8

Leishmania infantum BRC1 repeat in complex with LiRAD51

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-04-08
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9159
Spacegroup name	P 41 21 2
Unit cell lengths	61.004, 61.004, 119.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.310 - 2.150
R-factor	0.2791
Rwork	0.278
R-free	0.30560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n0w
RMSD bond length	0.012
RMSD bond angle	1.514
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.610	2.180
High resolution limit [Å]	2.150	2.150
Number of reflections	12826	632
<I/σ(I)>	15.6
Completeness [%]	98.9	98.9
Redundancy	23.1	18.1
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	32% low-PEG-smear, 0.1M Tris pH 8.5