7QUT

serial synchrotron crystallographic structure of Drosophila Melanogaster (6-4) photolyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2021-04-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.3
Spacegroup name	P 41
Unit cell lengths	103.600, 103.600, 52.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.300 - 2.240
R-factor	0.1522
Rwork	0.150
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ayv
RMSD bond length	0.009
RMSD bond angle	1.695
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.300	2.260
High resolution limit [Å]	2.240	2.240
Number of reflections	26853	1077
<I/σ(I)>	8.58
Completeness [%]	100.0
Redundancy	1948
CC(1/2)	0.990	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	277.15	100 mM bis-tris pH=6.5, 200 mM lithium sulphate monohydrate, 22 % PEG 3350, 0.5 % Ethyl acetate