7QUT
serial synchrotron crystallographic structure of Drosophila Melanogaster (6-4) photolyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 293.15 |
Detector technology | PIXEL |
Collection date | 2021-04-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.3 |
Spacegroup name | P 41 |
Unit cell lengths | 103.600, 103.600, 52.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.300 - 2.240 |
R-factor | 0.1522 |
Rwork | 0.150 |
R-free | 0.19110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ayv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.695 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.300 | 2.260 |
High resolution limit [Å] | 2.240 | 2.240 |
Number of reflections | 26853 | 1077 |
<I/σ(I)> | 8.58 | |
Completeness [%] | 100.0 | |
Redundancy | 1948 | |
CC(1/2) | 0.990 | 0.200 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.5 | 277.15 | 100 mM bis-tris pH=6.5, 200 mM lithium sulphate monohydrate, 22 % PEG 3350, 0.5 % Ethyl acetate |