7QTP

Structural biology of the NS1 avian influenza protein subversion on the Scribble cell polarity module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	C 1 2 1
Unit cell lengths	58.535, 51.202, 27.868
Unit cell angles	90.00, 90.55, 90.00

Refinement procedure

Resolution	38.540 - 1.900
Rwork	0.276
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwc
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER (1.15.2-3472-000)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.540	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.050	0.230
Number of reflections	6533	627
<I/σ(I)>	17.74
Completeness [%]	99.5
Redundancy	6.6
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.5M Ammonium nitrate, 0.1M Sodium acetate trihydrate pH 4.6