7QTO

Structural biology of the NS1 avian influenza protein subversion on the Scribble cell polarity module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	I 1 2 1
Unit cell lengths	56.909, 57.009, 61.598
Unit cell angles	90.00, 117.37, 90.00

Refinement procedure

Resolution	30.740 - 3.500
R-factor	0.2437
Rwork	0.242
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwc
RMSD bond length	0.002
RMSD bond angle	0.551
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.740	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.130	0.210
Number of reflections	4238	429
<I/σ(I)>	6.5
Completeness [%]	97.9
Redundancy	3.1
CC(1/2)	0.970	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M Ammonium sulfate, 30% w/v PEG 4000