7QTC

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-3 (G206H, R207T, D210P, S211Q)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	Agilent SuperNova
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-14
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54056
Spacegroup name	P 21 21 21
Unit cell lengths	51.353, 75.039, 149.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.358 - 2.550
Rwork	0.202
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.009
RMSD bond angle	1.585
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.360	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.119	0.676
Rmeas	0.143	0.830
Number of reflections	17974	2215
<I/σ(I)>	7.2	2.1
Completeness [%]	92.7	95.3
Redundancy	2.8	2.5
CC(1/2)	0.983	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 6000, 0.2 M MgCl2 in 0.1M Tris-HCl pH 8.5